Enzymatic preparation of an immunostimulant, the disaccharide‐dipeptide, N‐acetyl‐β‐D‐glucosaminyl‐(1→4)‐N‐acetylmuramyl‐L‐alanyl‐D‐isoglutamine, from a bacterial peptidoglycan
- 1 September 1984
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 143 (2) , 359-362
- https://doi.org/10.1111/j.1432-1033.1984.tb08380.x
Abstract
The disaccharide-dipeptide N-acetyl-.beta.-D-glucosaminyl-(1 .fwdarw. 4)-N-acetylmuramyl-L-alanyl-D-isoglutamine was obtained by an enzymatic degradation of the peptidoglycan of Actinomadura R39. The peptidoglycan was hydrolyzed successively by the 3 following enzymes: lysozyme, DD-carboxypeptidase from Streptomyces albus G and .gamma.-D-glutamyl-meso-diaminopimelate endopeptidase I from Bacillus sphaericus 9602. The by-products of the last reaction were eliminated by successive ion-exchange and gel-permeation chromatographies. Both chemical analysis and mass spectrometry show that the resulting disaccharide-dipeptide is a pure compound.This publication has 23 references indexed in Scilit:
- Action d'endopeptidases de Bacillus sphaericus sur les peptidoglycanes bactériens et sur des fragments peptidoglycaniquesBiochimie, 1983
- Synthesis of O-(2-acetamido-2-deoxy-β-D-glucosyl)-(1→4)-N-acetylmuramoyl-L-alanyl-D-isoglutamine, the repeating disaccharide-dipeptide unit of the bacterial cell-wall peptidoglycanCarbohydrate Research, 1979
- Caracterisation d'une nouvelle endopeptidase specifique des liaisons gamma-d-glutamyl-l-lysine et gamma-d-glutamyl-(l)meso-diaminopimelate de substrats peptidoglycaniques, chez Bacillus sphaericus 9602 au cours de la sporulationEuropean Journal of Biochemistry, 1979
- Mise en evidence au cours de la sporulation deBacillus sphaericusd'une activite dipeptidasique sur des substrats constituants du peptidoglycaneFEMS Microbiology Letters, 1978
- Relation between inhibition of Bacilli sporulation and synthesis of lytic enzymesBiochemical and Biophysical Research Communications, 1978
- Specificity Profiles of the Membrane‐Bound γ‐d‐Glutamyl‐(l)meso‐diaminopimelate Endopeptidase and ld‐Carboxypeptidase from Bacillus sphaericus 9602European Journal of Biochemistry, 1977
- Mise en évidence et séparation d'une γ-d-glutamyl-(l)meso-diaminopimélate endopeptidase et d'une ld-carboxypeptidase exocellulaires à partir de Bacillus sphaericus 9602Biochimie, 1977
- Préparation enzymatique de peptides du type (L)meso-diaminopimélyl(L)-D-Ala[14C]Biochimie, 1976
- Structure of the wall peptidoglycan of Streptomyces R39 and the specificity profile of its exocellular DD-carboxypeptidase-transpeptidase for peptide acceptorsBiochemistry, 1973
- Penicillin-sensitive DD-carboxypeptidases from Streptomyces strains R39 and K11Biochemistry, 1972