Molecular Cloning, Refined Physical Map and Heterogeneity of Methylation Sites of Papilloma Virus Type 1a DNA
- 1 August 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 109 (2) , 457-461
- https://doi.org/10.1111/j.1432-1033.1980.tb04815.x
Abstract
The entire genome of human papilloma virus type 1a was cloned in Escherichia coli using the plasmid pBR322 as vector. The integrity and the homogeneity of the viral DNA thus obtained was confirmed by restriction endonucleases analysis. Viral DNA isolated from a single wart was partially methylated at only 1 of the 4 HpaII sites, d(C-C-G-G). Recognition sites for BglI, BglII, PstI and PvuII restriction endonucleases were located on the cloned genome.This publication has 24 references indexed in Scilit:
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