cDNA cloning of functional T cell receptor γ/δ chains expressed in human peripheral blood lymphocytes

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Abstract
We have identified in earlier studies two V.delta. rearrangements corresponding to a 4.5-kb Eco RI fragment detected with a V.delta.1 probe and to a 7-kb Eco RI band detected with a V.delta.2 probe. These rearrangements have been found in two human T cell clones, F6C7 and G6, displaying surface phenotypes unfrequent in human peripheral blood, namely Ti.gamma.A+ BB3- (F6C7) and Ti.gamma.A- BB3+ (G6). Herein, we report the sequences of the functional transcripts encoded by these rearranged genes and show that the 4.5- and the 7-kb Eco RI fragments correspond to V1/D3/J.delta.3 and to V2/D3/J.delta.3 recombinations, respectively. In addition, we have sequenced the V2/D3/J1/C.delta. transcripts expressed in two clones, AB12 and VTC, which have a Ti.gamma.A+ BB3+ surface phenotype corresponding to that of most .gamma./.delta. peripheral lymphocytes. Analyses of the .delta. transcripts expressed by these four cells further strengthen the hypothesis that anti-BB3 and anti-.delta.-TCS-1 monoclonal antibodies recognize a V.delta.2- and a V1/(D)/J.delta.1-encoded epitope, respectively. Sequence of the .gamma. transcripts expressed by AB12 and F6C7 cells shows that they encode a V9/JP/C.gamma.1 chain. Finally, we confirm that non-combinatorial diversity in the .gamma. and .delta. proteins is generated by both junctional flexibility and N-region addition without any somatic mutation.

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