Photochemically Induced Gene Silencing Using PNA-Peptide Conjugates
- 1 June 2006
- journal article
- research article
- Published by Mary Ann Liebert Inc in Oligonucleotides
- Vol. 16 (2) , 145-157
- https://doi.org/10.1089/oli.2006.16.145
Abstract
The potential for exploration of peptide nucleic acid (PNA) as an experimental and therapeutic regulator of gene expression has been hampered by a poor delivery and a lack of site-specific targeting. In the present study, we have developed an efficient strategy for nuclear delivery of PNA by combining cationically charged PNA-peptide conjugates and photochemical internalization (PCI) technology. When using the S100A4 gene as a model system, a consistent downregulation to around 10% remaining protein signal was obtained in three selected cell lines. Furthermore, a dose-dependent and time-dependent inhibition of the S100A4 protein was demonstrated. A main benefit of the strategy proposed is the possibility of site-specific targeting.Keywords
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