DETECTION OF CHROMOSOME ANEUPLOIDY IN INTERPHASE NUCLEI FROM HUMAN PRIMARY BREAST-TUMORS USING CHROMOSOME-SPECIFIC REPETITIVE DNA PROBES
- 15 October 1988
- journal article
- research article
- Vol. 48 (20) , 5825-5830
Abstract
We have used in situ hybridization with chromosome specific repetitive DNA sequence as a probe to reveal particular chromosome as distinct spots or clusters of signal within interphase nuclei. Using karyotypically defined cells and cells lines, we show that the number of signals obtained per nucleus correlates with the number of particular chromosomes present in that nucleus. Further, admixtures of karyotypically different cell lines could be detected. In situ hybridization of nuclei and metaphase spreads derived from the breast cancer cell line MCF-7 shows that a deviant number of spots/nucleus indicates a numerical and/or structural chromosomal aberration. In seven primary breast tumors studied, we detected numerical aberrations of the target sites of chromosomes 1 and/or 18. Although all had a single peak in DNA flow measurements, six of the cases appeared to be heterogenous with respect to their spots/nucleus content.This publication has 14 references indexed in Scilit:
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