Studies on the Control of Fatty Acid Synthesis in White Adipose Tissue of the Rat: Effect of Alanine

Publisher Website
Google Scholar
Abstract
(1) Alanine resulted in inhibition of lipogenesis in adipocytes from normal fed rats incubated with glucose and insulin.(2) Two related mechanisms are suggested for the lower rates of lipogenesis caused by alanine metabolism. (a) Lower pyruvate levels were seen in the presence of alanine. This could contribute to the observed inhibition as pyruvate is an intermediate in the fatty acid synthesis pathway. Pyruvate levels were probably lowered as a result of the increased cytosol redox potential produced by high flow rates through malate dehydrogenase in this compartment, (b) Inhibition could be a consequence of depletion of malate in the cytosol which could limit the mitochondrial tricarboxylate carrier associated with citrate entry into the cytosol.(3) TMPD, an agent which lowers cytoplasmic redox potential, increased both the malate and pyruvate levels and increased the lipogenic rates nearly to normal in the presence of alanine.

This publication has 0 references indexed in Scilit: