Identification of a telomere-binding activity from yeast.
- 1 June 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (11) , 3713-3717
- https://doi.org/10.1073/pnas.83.11.3713
Abstract
In yeast, the ends of the chromosomes (telomeres) terminate in repeated poly(C1-3A) sequences. We have identified a yeast activity that binds specifically to these poly(C1-3A) repeats. An agarose gel binding assay was used to detect and characterize this activity in cell extracts using both cloned telomere DNA and yeast genomic DNA as substrates. The activity appears to bind specifically to poly(C1-3A) sequences, despite their different primary sequences, yet does not bind specifically to telomeric repeats, such as poly(C4A2), poly(C4A4), and poly (C1-8T) from other lower eukaryotes.Keywords
This publication has 47 references indexed in Scilit:
- Identification of a specific telomere terminal transferase activity in tetrahymena extractsCell, 1985
- Regulation of the Escherichia coli l-arabinose operon studied by gel electrophoresis DNA binding assayJournal of Molecular Biology, 1984
- Chromatin structure of the molecular ends of oxytricha macronuclear DNA: phased nucleosomes and a telomeric complexCell, 1984
- Unusual DNA sequences associated with the ends of yeast chromosomesNature, 1984
- Kinetics and mechanism in the reaction of gene regulatory proteins with DNAJournal of Molecular Biology, 1984
- Equilibrium studies of the cyclic AMP receptor protein-DNA interactionJournal of Molecular Biology, 1984
- Is there left-handed DNA at the ends of yeast chromosomes?Nature, 1983
- A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in TetrahymenaJournal of Molecular Biology, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Sugar and DiseaseNature, 1972