High-Performance Liquid Chromatographic Determination of Trace Quantities of Azinphos Methyl and Azinphos Methyl Oxon in Various Water Sources by Direct Injection and Trace Enrichment

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Abstract
A high-performance liquid chromatographic (HPLC) method is described for the determination of trace amounts of the organophosphate insecticide, azinphos methyl and its degradation product, azinphos methyl oxon, by direct injection and by trace enrichment. The compounds were analyzed on a uBondapak C18 column with UY detection at 224 nm. The mobile phase for the analysis was acetonitrile-water (50:50) at a flow rate of 1.3 ml/min. Ten minutes were required for the chromatographic analysis. Water from three sources, public water supply, stream, and ocean was analyzed for azinphos methyl and azinphos methyl oxon at concentrations as low as 11.9 and 11.3 ppb, respectively, without a clean-up, concentration or derivatization step. Azinphos methyl was quantitated at 0.29 ppb and azinphos methly oxon at 0.29 ppb by employing a concentration step involving a C18 Sep-Pak cartridge. The coefficients of variation for all determinations ranged from 0.77 to 9.06%. Peak heights were used for quanti-tation. Several other pesticides have been shown not to interfere with the analysis of either compound.

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