Cloning and sequencing of the ura3 locus of the methylotrophic yeast Hansenula polymorpha and its use for the generation of a deletion by gene replacement
- 1 November 1993
- journal article
- applied genetics-and-regulation
- Published by Springer Nature in Applied Microbiology and Biotechnology
- Vol. 40 (2-3) , 361-364
- https://doi.org/10.1007/bf00170393
Abstract
The ura3 gene of Hansenula polymorpha was cloned, sequenced and used to generate a ura3 mutant from the wild-type strain of this yeast via integrative mutagenesis. The Tn5 neomycin-resistance marker (neo) under control of the ADH1 promoter from Saccharomyces cerevisiae served as a transformation marker. The results show that gene replacement can be achieved in H. polymorpha, a yeast with a high level of non-homologous integration.Keywords
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