Isolation of Polysomes from Nostoc sp. MAC and Translation of Messenger RNA in a Heterologous Cell-free System
- 1 August 1983
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 129 (8) , 2359-2366
- https://doi.org/10.1099/00221287-129-8-2359
Abstract
SUMMARY: A method has been established which isolates polysomes from the lysozyme/EDTA-shocked cyanobacterium, Nostoc sp. MAC. In a typical preparation the total recovery of RNA as polysomes was 83%, in which 77% of the polysome fraction was present at sizes > 5-mers and 23% as 2-4-mers. Messenger RNA isolated from such a preparation of polysomes produced a 10-fold stimulation in the incorporation of [35S]methionine into polypeptides by a cell-free system of Escherichia coli. The in vitro-synthesized polypeptides were analysed on an SDS-polyacrylamide gradient gel together with in vivo-labelled proteins of Nostoc sp. MAC: seven polypeptides co-migrated with the in vivo-synthesized products. This is the first report of the expression of cyanobacterial messenger RNA in a heterologous cell-free system from E. coli; the efficiency of the system is discussed.Keywords
This publication has 1 reference indexed in Scilit:
- Phycocyanins: Structure and FunctionPublished by Springer Nature ,1976