The Types of Rat Thoracic Duct Lymphocytes which Respond to Phytohemagglutinin in vitro

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Abstract
Radio-autographic identification of the cells responding to phytohemagglutinin (PHA) was accomplished in cultures of thoracic duct lymphocytes from rats injected with H3-thymidine according to schedules which label mainly short-lived or only long-lived small lymphocytes. Both long-and short-lived small lymphocytes enlarge and divide when stimulated by PHA, but for a given number of cells from either population, shortlived are 4 to 6 times more responsive. Large and medium lymphocytes preexisting in culture inocula make only an insignificant contribution to enlarged forms developing during culture. The relatively heavy average labeling noted in short-lived small lymphocytes as well as the facility with which they proliferate in vitro suggest that these cells may be most active in normal proliferative cycles in vivo.

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