An Incubation Method for the Estimation of Urinary Kallikrein

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Abstract
An incubation method for the estimation of urinary kallikrein is described. It consists of incubation of urine with heated human plasma substrate (56 58C, 3 hrs) and assay of formed kinin on the guinea-pig ileum against synthetic brady-kinin as a standard. Kallikrein content of the urine is calculated by comparing its kinin forming activity with that of standard urine. Twenty-four hour excretions of urinary kallikrein were 23-600 Frey units in total 58 individuals including both healthy and variously diseased subjects. These values are in good accordance with those of former authors who estimated kallikrein on dog''s blood pressure. No significant difference was observed in kallikrein excretion between various diseases.

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