Volume‐activated Cl− secretion and transepithelial vinblastine secretion mediated by P‐glycoprotein are not correlated in cultured human T84 intestinal epithelial layers
- 15 June 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 304 (2-3) , 233-236
- https://doi.org/10.1016/0014-5793(92)80626-r
Abstract
The relationship between the P‐glycoprotein‐mediated vinblastine secretion and cell‐swelling activated Cl− secretion (conductance) in intact epithelial layers of human colonic adenocarcinoma T84 cells has been investigated. Whereas vinblastine secretion is effectively inhibited by 100 μM 1,9‐dideoxy‐forskolin, volume‐stimulated Cl− secretion is unaffected. In contrast, 100 μM 4,4′‐diisothiocyanostilbene‐2,2′disulphonic acid (DIDS) inhibited the volume‐stimulated Cl− secretion, but was without effect upon transepithelial vinblastine secretion. In addition, it was noted that some epithelial layers failed to express a volume‐stimulated Cl− secretion but maintained a normal level of secretory vinblastine flux.Keywords
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