Sensitive Determination of Cisplatin in Body Fluids with HPLC and On-Line Reductive Electrochemical Detection

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Abstract
A sensitive method for the determination of the antitumor agent cisplatin has been developed consisting of solvent-generated anion exchange chromatography and on-line reductive electrochemical detection. CDDP was retained on a hexadecyl-trimethylammonium-loaded reversed phase HPLC column using a 5mM citrate-buffered eluent (pH 6.5). Exploiting adsorption of CDDP at a dropping mercury electrode and NH4+-enhanced catalytic proton reduction, a detection limit of 10−8 M (3 ng/ml) has been achieved. Application of the developed method to the quantification of CDDP in plasma ultrafiltrate and urine is demonstrated and discussed.