MICROSOMAL N-HYDROXYLATION OF TRANS-4'-HALO-4-ACETAMIDOSTILBENES

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 5  (3) , 310-316
Abstract
Hamster liver microsomes catalyze the N-hydroxylation of trans-4-acetamidostilbene and its 4''-Cl and 4''-F analogs. Other metabolites which were detected and quantitated were the 4''-OH derivative of trans-4-acetamidostilbene and the glycolamides of the 4''-Cl and 4''-F compounds. The kinetics of metabolism of the acetamidostilbenes were also determined. The apparent Km values of N-hydroxylation of the 4 substrates were similar, but the maximal velocities showed marked variations. The Vmax for the 4''-Br analog was 16% of that of trans-4-acetamidostilbene, and the rate of N-hydroxylation of the 4''-Cl and 4''-F analogs was only 3% of that of the parent compound.