He-Ne laser irradiation affects proliferation of cultured rat Schwann cells in a dose-dependent manner

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Abstract
Summary Schwann cell proliferation is considered an essential part of Wallerian degeneration after nerve damage. Laminin, an important component of the extracellular matrix and produced by Schwann cells, provides a preferred substrate for outgrowing axons. To study whether low energy (He-Ne) laser irradiation may exert a positive effect on nerve regeneration through an effect on Schwann cells, its effect was evaluatedin vitro. Schwann cells were isolated from sciatic nerves of 4–5-day-old Wistar rates and cultured on 96-multiwell plates. The cells were irradiated by a He-Ne laser beam (632.8 nm, 5.98 mW) that was optically expanded to a beam width of 4 mm. During irradiation the plate was kept in an air-tight box equilibrated with humidified air containing 5% CO2 and kept at 37 ° C. At three consecutive days, starting either at day 5 or day 8, cells were irradiated each day for 0.5, 1, 2, 5 or 10 min. Both cell number and laminin production were determined for each irradiation condition (n=5) within one experiment. Schwann cells that were irradiated from day 8 on were hardly affected by laser irradiation. However, the proliferation of cells that were irradiated starting on day 5 was significantly increased after 1, 2, and 5 min of daily irradiation, compared to non-irradiated control cultures. The laminin production per cell of these Schwann cells was not significantly altered. From these results we conclude that He-Ne laser irradiation can modulate proliferation of rat Schwann cellsin vitro in a dose-dependent manner.