Immunochemical Studies of Human Urinary Erythropoietin.

Abstract
Experiments indicate that the erythropoietic activity as assayed in the polycythemic mouse of erythropoietically active human urinary concentrates can be neutralized in vitro by the addition of sera obtained from rabbits previously immunized with such concentrates. Injection of such sera into normal mice depresses the 24-hour Fe59 uptake to 8.7% compared to 21% for saline injected animals. It is concluded that this neutralization is the result of an antigen-antibody reaction directed against either the erythropoietically active molecule or the general class of proteins to which this molecule belongs.

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