GENERATION OF EXPRESSED SEQUENCE TAGS FROM A NORMALIZED PORCINE SKELETAL MUSCLE cDNA LIBRARY
- 1 December 2002
- journal article
- Published by Taylor & Francis in Animal Biotechnology
- Vol. 13 (2) , 211-222
- https://doi.org/10.1081/abio-120016190
Abstract
Recent developments in microarray technologies permit scientists to analyze expression of thousands of genes simultaneously in diverse biological systems. In an effort to provide integrated resources for application of microarray technologies to studies of skeletal muscle growth and development in swine, we have constructed a normalized cDNA library from porcine skeletal muscle. The effectiveness of normalization was evaluated by DNA sequencing of clones randomly picked from the library before and after normalization, and also by Southern blot hybridization using probes representing abundant transcripts. Our data suggests that the normalization procedure successfully reduced the highly abundant cDNA species in the normalized library. To date, a total of 782 EST (expressed sequence tag) sequences have been generated from this normalized library (687 ESTs) and the original library (95 ESTs). The sequence information of these ESTs plus their BLAST results has been made available through a web access...Keywords
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