DEVELOPMENT OF MICRO-AGGLUTINATION TEST FOR DETECTING ARIZONA-HINSHAWII-7=1,7,8 INFECTION IN TURKEYS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (2) , 255-257
Abstract
A microagglutination antigen treated with tetrazolium dye was produced. The culture used was rendered nonmotile by growing in medium containing 1:1000 concentration of phenol. A trial was conducted by infecting freshly hatched turkey poults with A. hinshawii 7:1,7,8 [Salmonella arizonae]. An equal number of poults were kept as negative controls. Starting with 4 wk old poults, blood samples, cloacal swabs and environmental samples were obtained at 2 wk intervals up to 16 wk old. The serum samples were tested by using tube-agglutination (TA), serum plate agglutination (SPA) and microagglutination (MA) tests for A. hinshawii 7:1,7,8. A field trial was conducted using known negative and infected turkey flocks, and serum samples were tested by MA, SPA and TA tests. The MA test was far more sensitive and superior to TA and SPA tests in detecting turkeys infected with A. hinshawii 7:1,7,8. The field trial confirmed the laboratory trial observations.

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