Self‐assembly of ATP synthase subunit c rings
- 4 March 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 515 (1-3) , 189-193
- https://doi.org/10.1016/s0014-5793(02)02447-x
Abstract
Subunit c of the H+ transporting ATP synthase is an essential part of its membrane domain that participates in transmembrane proton conduction. The annular architecture of the subunit c from different species has been previously reported. However, little is known about the type of interactions that affect the formation of c‐rings in the ATPase complex. Here we report that subunit c over‐expressed in Escherichia coli and purified in non‐ionic detergent solutions self‐assembles into annular structures in the absence of other subunits of the complex. The results suggest that the ability of subunit c to form rings is determined by its primary structure.Keywords
This publication has 33 references indexed in Scilit:
- Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over‐production of the b subunit of F1Fo ATP synthaseFEBS Letters, 2000
- Insights into the Rotary Catalytic Mechanism of F0F1 ATP Synthase from the Cross-linking of Subunits b and c in the Escherichia coli EnzymePublished by Elsevier ,2000
- F‐ATPase: specific observation of the rotating c subunit oligomer of EFoEF1FEBS Letters, 2000
- The γ subunit in chloroplast F1‐ATPase can rotate in a unidirectional and counter‐clockwise mannerFEBS Letters, 1999
- Molecular Architecture of the Rotary Motor in ATP SynthaseScience, 1999
- The γε-c Subunit Interface in the ATP Synthase ofEscherichia coliJournal of Biological Chemistry, 1999
- Solution Structure of the Transmembrane H+-Transporting Subunit c of the F1Fo ATP SynthaseBiochemistry, 1998
- Topographical structure of membrane‐bound Escherichia coli F1F0 ATP synthase in aqueous bufferFEBS Letters, 1996
- Over-production of Proteins inEscherichia coli: Mutant Hosts that Allow Synthesis of some Membrane Proteins and Globular Proteins at High LevelsJournal of Molecular Biology, 1996
- SPIDER and WEB: Processing and Visualization of Images in 3D Electron Microscopy and Related FieldsJournal of Structural Biology, 1996