A DETACHED LEAF TECHNIQUE FOR SCREENING BEANS (Phaseolus vulgaris L.) IN VITRO AGAINST ANTHRACNOSE (Colletotrichum lindemuthianum)

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Abstract
An effective alternative method of screening white beans (Phaseolus vulgaris L.) for anthracnose (Colletotrichum lindemuthianum (Sacc. & Magn.) Briosi & Cav.) resistance was developed using excised leaves or leaflets. The inoculum was brushed on the underside of the excised leaves and leaflets. The inoculated leaves were placed, inoculated side up, on several layers of wet paper towels in a tray which was then covered with a transparent lid or plastic to maintain high humidity. The trays were kept at 22 ± 1 °C with 9 h light daily for 6 d for symptom development. With this method one plant can be assayed several times either for the same race or for different races of the pathogen. The latter is particularly important when resistance to different races involves different genes.Key words: Bean (navy), anthracnose, screening technique

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