A study of the kinetics and mechanism of rabbit muscle l-glycerol 3-phosphate dehydrogenase

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Abstract
1. The kinetics of oxidation of l-glycerol 3-phosphate by NAD+and of reduction of dihydroxyacetone phosphate by NADH catalysed by rabbit muscle glycerol 3-phosphate dehydrogenase were studied over the range pH6–9. 2. The enzyme was found to catalyse the oxidation of glyoxylate by NAD+at pH8.0 and the kinetics of this reaction were also studied. 3. The results are consistent with a compulsory mechanism of catalysis for glycerol 3-phosphate oxidation and dihydroxyacetone phosphate reduction in the intermediate regions of pH, but modifications to the basic mechanism are required to fully explain results at the extremes of the pH range, with these substrates and for glyoxylate oxidation at pH8.0.