1′-Acetoxychavicol Acetate, a Superoxide Anion Generation Inhibitor, Potently Inhibits Tumor Promotion by 12-O-Tetradecanoylphorbol-13-Acetate in ICR Mouse Skin
- 1 January 1996
- journal article
- laboratory investigation
- Published by S. Karger AG in Oncology
- Vol. 53 (5) , 386-391
- https://doi.org/10.1159/000227593
Abstract
The anti-tumor-promoting activity of 1′-acetoxychavicol acetate (ACA) was examined in a two-stage carcinogenesis experiment in ICR mouse skin using 7,12-dimethylbenz[a]anthracene (0.19 µmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA; 1.6 nmol). Topical application of ACA (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice: inhibition ratios 90% (p < 0.001) and 42% (p < 0.005), respectively. ACA even at a dose equimolar to TPA (1.6 nmol) significantly reduced the average number of tumors per mouse: inhibitory ratio 44% (p < 0.05). ACA potently inhibited TPA-induced superoxide (O2) generation in differentiated HL-60 cells (IC50 = 4.3 µM) and suppressed the lipid hydroperoxide formation by 42% (p < 0.001) in the ethyl linoleate autoxidation test.Keywords
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