DNA Polymerase-Mediated DNA Synthesis on a TNA Template
Open Access
- 28 December 2002
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (4) , 856-857
- https://doi.org/10.1021/ja028589k
Abstract
TNA, or threose nucleic acid, is capable of Watson−Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.Keywords
This publication has 17 references indexed in Scilit:
- The antiquity of RNA-based evolutionNature, 2002
- A Simpler Nucleic AcidScience, 2000
- Chemical Etiology of Nucleic Acid Structure: The α-Threofuranosyl-(3'→2') Oligonucleotide SystemScience, 2000
- Oligonucleotide aptamers that recognize small moleculesPublished by Elsevier ,1999
- Chemical Etiology of Nucleic Acid StructureScience, 1999
- Determination of the site of first strand transfer during Moloney murine leukemia virus reverse transcription and identification of strand transfer-associated reverse transcriptase errorsThe EMBO Journal, 1997
- Randomization of genes by PCR mutagenesis.Genome Research, 1992
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA PolymeraseScience, 1990
- Effect of manganese ions on the incorporation of dideoxynucleotides by bacteriophage T7 DNA polymerase and Escherichia coli DNA polymerase I.Proceedings of the National Academy of Sciences, 1989