Radiation Inactivation of T7 Phage

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Abstract
The radiation inactivation of T7 phage by 25 MeV electron pulses was measured in various media containing a wide concentration range of radical savenging solutes and in the presence of protective and sensitizing agents. The dependence of sensitivity on pulse dose, from 1 mrad to 3.6 krad, is attributed to radical depletion via bimolecular processes. The survival data are analyzed by extending target theory to include diffusive reactions of primary and secondary radicals generated in the medium. OH radicals are the principal primary inactivating species and secondary radicals from Br-, CNS-, uracil, glucose, ribose, sucrose, tyrosine and histidine are lethal to some extent. In nutrient broth or 100 mM histidine, psoralen derivatives, actinomycin D and mitomycin C are anoxic sensitizers. Apparently, psoralens promote the formation of non-strand break lesions as the sensitization mechanism. The target theory analysis based on diffusional kinetics is applicable to other systems including single cells.

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