A novel drug delivery system for osteomyelitis using porous hydroxyapatite blocks loaded by centrifugation

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Abstract
A novel drug delivery system for osteomyelitis was developed using porous hydroxyaptite blocks (HA‐b) loaded with antibodies by centrifugation. In the study, 10 mm3 HA‐b was placed in a container and mixed with antibiotic solution; the antibiotic was then loaded into the pores of the HA‐b by centrifuging at 1500 rpm for 15 min. Slow release of HA‐b in both moist form and dried form (by heating at 160°C) was tested after loading with the antibiotic arbekacin sulfate (ABK), 1‐N‐(S)‐4 amino‐2‐hydroxybutyryl dibekacin. To estimate the concentration of antibiotic, both forms of HA‐b were placed in 3 mL of phosphate buffered saline (PBS), which was replaced every 48 h. In both groups, which were loaded with 70 mg ABK per one block of HA (concn 0.5 μg/mL) which is sufficiently high to control most pathogens, was maintained for 21 exchanges of PBS (after 42 days). Minimum inhibitory concentration for methicillin‐resistant Staphylococcus aureus (MRSA), 3.13 μg/mL, was maintained until nine exchanges took place (after 18 days). The centrifugation method is wsimple, and driked ABK produced by heating loaded HA‐b is particularly useful in clinical applications for osteomyelitis. © 1995 John Wiley & Sons, Inc.