Embedding with Low Viscosity Nitrocellulose

Publisher Website
Google Scholar
Abstract
The gelation and cutting of embedding masses of low viscosity nitrocellulose (L. V. N.) and of celloidin were compared. L. V. N. forms a firmer mass which can be cut into thinner sections than celloidin. It tolerates considerable water (up to 6%) in a solvent system of alcohol-water-ether, thereby permitting the use of 95% alcohol instead of absolute for making up the solution. The fluidity of its solutions permits transfer of tissue directly from alcohol-ether to a 20% embedding solution. Faults of L. V. N. are: the nitrated cotton lint contains some grit (hence its solution should be allowed to settle), the sections cut from it are more easily torn than celloidin sections, and it is sufficiently soluble in absolute alcohol to preclude the use of this fluid in handling sections.

This publication has 0 references indexed in Scilit: