Further studies on the biological activity of bulbiformin

Abstract
SUMMARY: Amendment of soil with roots of certain leguminous crops, molasses and oil cakes markedly increased the antibiotic production by Bacillus subtilis. A soil amendment consisting of a combination of groundnut cake and molasses was about five times more effective than a dextrose amendment in increasing the production of bulbiformin and also favoured its persistence in the soil. The antibiotic was found to act systemically and to be non‐phytotoxic when taken up by the roots of pigeon pea (Cajunus cajan).In a pot experiment, inoculation of B. subtilis into autoclaved soil amended with molasses, sweet clover roots and groundnut cake brought about a reduction of 88% in the incidence of pigeon pea wilt caused by Fusarium udum.