Abstract
Intracellular recordings were made from neurones of the rat locus coeruleus in vitro. Acetylcholine increased the firing rate by depolarizing the membrane; both muscarinic and nicotinic antagonists partially reduced this effect. In hexamethonium, pirenzepine shifted the acetylcholine dose‐response curve to the right with an equilibrium dissociation constant of 200 nM, indicating an interaction with a m2‐type of muscarinic receptor.