Glycollate formation during the photoassimilation of acetate by Chlorella

Abstract
When 3H-14C-acetate was supplied to Chlorella pyrenoidosa in the light, glycollic acid became rapidly labelled with tritium and 14C. The \(\frac{{{}^3{\text{H}}}}{{{}^{14}{\text{C}}}}\) ratio of glycollate was 10, whilst the ratio was 4 in the acetate added. Both 3H and 14C from acetate were present in glycollate before they were present in Calvin cycle intermediates, so that glycollate was not formed as a C2-fragment from the Calvin cycle.