Influence of Age and Dietary Stress on Hexobarbital Activity in Mice.

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Abstract
Age and diet markedly influence drug detoxlcatlon in mice. In this work, CF-1 male mice were maintained on semi-synthetic, isocaloric protein diets of 8% or 27% casein from weaning to early maturity. The mice fed the 8% casein diet slept longer after administration of 100 mg/kg intraperitoneally of hexo-barbital than did the mice fed the 27% casein diet at all time periods tested. The 9, 000 X G liver supernatants from mice fed the 8% casein diet metabolized hexobarbital to a significantly lower degree than did this same fraction from the mice fed the 27% casein diet. During the period of rapid body growth, the protein deficient mice were capable of synthesizing hexobarbital metabolizing enzyme. The prolonged sleeping time observed during the early periods on the diet was believed to be due to an insufficient supply of TPNH. During the 4th to the 5th week on the 8% casein diet, the marked prolongation in sleeping time seen was thought to be due to an actual decrease in drug metabolizing enzyme and cofactor, as well as to a decrease in body fat level.

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