Isolation and characterization of a new zinc-binding protein from albacore tuna plasma
- 2 June 1987
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 26 (11) , 3228-3234
- https://doi.org/10.1021/bi00385a044
Abstract
The protein responsible for sequestering high levels of zinc in the plasma of the albacore tuna (Thunnus alalunga) has been isolated by sequential chromatography. The glycoprotein has a molecular weight of 66,000. Approximately 8.2% of its amino acid residues are histidines. Equilibrium dialysis experiments show it to bind 3 mol of zinc/mol of protein. The stoichiometric constant for the association of zinc with a binding site containing three histidines was determined to be 109.4. This protein is different from albumin and represents a previously uncharacterized zinc transport protein.Keywords
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