Modelling the dynamics of the yeast pheromone pathway
Open Access
- 30 July 2004
- Vol. 21 (10) , 831-850
- https://doi.org/10.1002/yea.1122
Abstract
We present a mathematical model of the dynamics of the pheromone pathways in haploid yeast cells of mating type MATa after stimulation with pheromone α‐factor. The model consists of a set of differential equations and describes the dynamics of signal transduction from the receptor via several steps, including a G protein and a scaffold MAP kinase cascade, up to changes in the gene expression after pheromone stimulation in terms of biochemical changes (complex formations, phosphorylations, etc.). The parameters entering the models have been taken from the literature or adapted to observed time courses or behaviour. Using this model we can follow the time course of the various complex formation processes and of the phosphorylation states of the proteins involved. Furthermore, we can explain the phenotype of more than a dozen well‐characterized mutants and also the graded response of yeast cells to varying concentrations of the stimulating pheromone. Copyright © 2004 John Wiley & Sons, Ltd.Keywords
This publication has 80 references indexed in Scilit:
- Regulation of MAPK Function by Direct Interaction with the Mating-Specific Gα in YeastScience, 2002
- Pheromone-dependent Ubiquitination of the Mitogen-activated Protein Kinase Kinase Ste7Published by Elsevier ,2002
- The Complete Pathway for Catalytic Activation of the Mitogen-activated Protein Kinase, ERK2Published by Elsevier ,2001
- MAP kinase dynamics in response to pheromones in budding yeastNature Cell Biology, 2001
- Emergent Properties of Networks of Biological Signaling PathwaysScience, 1999
- Membrane recruitment of the kinase cascade scaffold protein Ste5 by the Gβγ complex underlies activation of the yeast pheromone response pathwayGenes & Development, 1998
- Functional binding between Gβ and the LIM domain of Ste5 is required to activate the MEKK Ste11Current Biology, 1998
- Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiaeCell, 1994
- Phospholipase C-β1 is a GTPase-activating protein for Gq/11, its physiologic regulatorCell, 1992
- Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2Cell, 1990