Growth of Micro-organisms in Gel-stabilized Two-dimensional Diffusion Gradient Systems

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Abstract
The Szybalski wedge plate technique was modified to generate 2-dimensional diffusion gradients for 2 environmental variables: pH value and NaCl concentration were selected in this study. After experimentation, pH values chosen ranged from 3.9-8.1 and salt concentrations from 23-79 gl-1. The pH gradients were slightly sigmoidal while the salt gradients were approximately linear. The reproducibility of the technique was assessed; it was satisfactory in replicate growth experiments. A wide range of growth patterns was observed for a large variety of different bacterial types. The growth boundary and the pattern details are sufficiently distinct to suggest that the technique can distinguish between closely related species or strains.

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