Dynamic Combinatorial Mass Spectrometry Leads to Metallo-β-lactamase Inhibitors
- 19 January 2008
- journal article
- Published by American Chemical Society (ACS) in Journal of Medicinal Chemistry
- Vol. 51 (3) , 684-688
- https://doi.org/10.1021/jm070866g
Abstract
The use of protein ESI mass spectrometry under non-denaturing conditions to analyze a dynamic combinatorial library of thiols/disulfides with the BcII metallo-beta-lactamase enabled the rapid identification of an inhibitor with a K(i) of < 1 microM. The study exemplifies the utility of protein-MS for screening dynamic mixtures of potential enzyme-inhibitors.Keywords
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