Studies on Luciferase from Photobacterium phosphoreum
- 1 October 1972
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 72 (4) , 951-955
- https://doi.org/10.1093/oxfordjournals.jbchem.a129986
Abstract
A new flavin derivative (p-flavin) was isolated from a bacterial luciferase from Photobacterium phosphoreum. It contains isoalloxazine nucleus and phosphoric acid in a ratio of 1:4, but is more hydrophobic than FMN or riboflavin and is soluble in chloroform. The chromatographic characteristics of this new flavin do not correspond to those of any previously known flavin derivatives. Its absorption maxima are at 224, 270, 385, and 445 nm with a shoulder at 470 nm, and its fluorescence maximum (absolute spectrum) is at 544 nm, in aqueous medium at pH7.0. One molecule of native luciferase was found to contain an average of 0.19 molecule of this flavin, bound non-covalently.Keywords
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