A MICRO–METHOD FOR THE ESTIMATION OF KHELLIN IN PRESENCE OF OTHER CONSTITUENTS OFAMMI VISNAGAFRUITS

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Abstract
For separation of khellin from other constituents of the fruit, alcoholic and petroleum ether extracts were applied on kiesel gel (G) plates and developed with solvent system ethyl acetate : benzene : water (50:75:50). This system proved to be effective in separation of khellin from visnagin, coumarins, glycosides, chlorophyll and other extraneous material. A micro method for estimating the separated khellin is described, depending on eluting khellin whit 4 cc of hot distilled water, adding 5 cc of 20 N sulfuric acid and volume adjusted to 10 cc, the colour developed measured after 5 minutes at 420 [xm. The reproducibility of the method was proved by confirming hundred percent elution of known quantities of authentic khellin. Khellin ist von anderen Inhaltsstoffen der Früchte von Ammi visnaga (z. B. Visnagin, Chromone, Cumarine, Glykoside, Chlorophyll u. a.) durch DC auf Kieselgel G mit dem Laufmittel Aethylacetat/Benzol/Wasser (50:75:50) abtrennbar. Das abgetrennte Khellin kann von der DC–Platte eluiert und durch anschließende kolorimetrische Messung quantitativ bestimmt werden.

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