Identification by C‐banding of two human prostate tumour cell lines, 1013L and DU 145

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Abstract
Two human prostate carcinoma cell lines are easily distinguished by C‐banding. DU 145 has one metacentric chromosome with a large centromeric band, I metacentric and 2 acrocentric chromosomes with interstitial C‐bands. The Y chromosome was present in 48% and 42% of the cells at passages 83 and 106, respectively, whereas in I0I3L only one metacentric chromosome contained distinct extra C bands—one at the centromere and one interstitially. The Y chromosome was present in 72% of the cells at passage 15 and in 20% at passage 22, but by passage 44 it had disappeared. Karyotype analysis using G‐banding revealed that DU 145 had retained several of its original markers, and demonstrated multiple marker chromosomes in 1013L.