Immobilization of immunoglobulins on polystyrene latex beads: Characterization by density gradient centrifugation

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Abstract
Isopycnic banding by density gradient centrifugation was used to measure density changes in complexes formed by the immobilization of each of four different immunoglobulins (IgG) (bovine, dog, rabbit, and sheep) on polystyrene latex beads (0.109 ± 0.0025 μm diam). Subtractive measurements of density changes allowed calculation of the mass of immobilized lgG under varying experimental conditions. The immobilization data were correlated with adsorption isotherms which incorporated charge repulsion forces. The effects of pH and NaCl concentration on the immobilization were studied for the latex–bovine lgG system. It was found that the mass of immobilized immunoglobulins was increased from 10 to 20% by removing the lgG from its isoelectric range.