Comparative studies on Salmonella typhi grown in vivo and in vitro II. The effect of extracts from normal and infected organs on the bactericidal serum action on strains grown in vivo and in vitro

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Abstract
The sensitivity of in vitro- and in vivo-grown strains of S. typhi was determined not only by their antigenic structure, but also by their growth phase. An increased vulnerability to the antibody-complement system has been found in cells during the lag and the early exponential phase, while non-multiplying cells devoid of any nutrient were almost invulnerable to the antibody-complement system. Extracts of organs from normal and infected animals may promote the growth of S. typhi, and, therefore, increase its vulnerability to the antibody-complement system. The majority of extracts from organs of mice infected with strain Ty 2 inhibited markedly the bactericidal action of serum on this strain and, to a lower extent, on strain O 901.