High Interlaboratory Reproducibility of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria
- 1 November 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (11) , 3732-3734
- https://doi.org/10.1128/jcm.00921-09
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry has emerged as a rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (using eight laboratories) achieved 98.75% interlaboratory reproducibility. Only 6 of the 480 samples were misidentified due to interchanges (4 samples) or contamination (1 sample) or not identified because of insufficient signal intensity (1 sample).Keywords
This publication has 10 references indexed in Scilit:
- Challenging the problem of clostridial identification with matrix-assisted laser desorption and ionization–time-of-flight mass spectrometry (MALDI–TOF MS)Anaerobe, 2008
- Evaluation of Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry in Comparison to 16S rRNA Gene Sequencing for Species Identification of Nonfermenting BacteriaJournal of Clinical Microbiology, 2008
- High Interlaboratory Reproducibility of DNA Sequence-Based Typing of Bacteria in a Multicenter StudyJournal of Clinical Microbiology, 2006
- Bacterial analysis by MALDI-TOF mass spectrometry: An inter-laboratory comparisonJournal of the American Society for Mass Spectrometry, 2005
- Intact cell mass spectrometry (ICMS) used to type methicillin-resistant Staphylococcus aureus: media effects and inter-laboratory reproducibilityJournal of Microbiological Methods, 2001
- Characterization of intact microorganisms by MALDI mass spectrometryMass Spectrometry Reviews, 2001
- Investigation of spectral reproducibility in direct analysis of bacteria proteins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometryRapid Communications in Mass Spectrometry, 1998
- The rapid identification of intact microorganisms using mass spectrometryNature Biotechnology, 1996
- Rapid Identification of Intact Whole Bacteria Based on Spectral Patterns using Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass SpectrometryRapid Communications in Mass Spectrometry, 1996
- Detection of Pathogenic and Non-pathogenic Bacteria by Matrix-assisted Laser Desorption/ Ionization Time-of-flight Mass SpectrometryRapid Communications in Mass Spectrometry, 1996