Simple Determination of Steroid Sulfatase Activity in Enzyme Preparation
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 14 (17-18) , 1515-1523
- https://doi.org/10.1080/00032718108081477
Abstract
Simple one tube method for the determination of steroid sulfatase activity in an enzyme preparation or commercially available preparation is described. Principle of the reaction is as follows; dehydroepi-androsterone sulfate (DHEA-S) as substrate is hydrolyzed by the catalysis of sulfatase and DHEA released is determined by the enzymic method using 3β-hydroxysteroid oxidase.Keywords
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