Antigen‐specific antibody response induced in cultures of human blood lymphocytes in presence of polyethylene glycol
- 1 January 1981
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 11 (11) , 943-945
- https://doi.org/10.1002/eji.1830111117
Abstract
Human peripheral blood lymphocytes respond in vitro to sheep red blood cells (SRBC) with the appearance of numerous specific plaque-forming cells, if the Epstein-Barr virus (EBV) is added to the cultures together with the antigen. However, it was found that when polyethylene glycol (mol. wt. 6000) is included in the culture medium at a final concentration of 4%, antigen alone is able to induce an anti-SRBC response, with clear hemolytic plaques of regular size. Addition of EBV causes a further increase in the antigen-specific antibody response.This publication has 15 references indexed in Scilit:
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