Oxidative stress in the pathogenesis of nonalcoholic fatty liver disease, in rats fed with a choline‐deficient diet

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Abstract
Background/Aim: The pathogenesis of Nonalcoholic Fatty Liver Disease remains largely unknown, but oxidative stress seems to be involved. The aim of this study was to evaluate the role of oxidative stress in experimental hepatic steatosis induced by a choline‐deficient diet.Methods: Fatty liver disease was induced in Wistar rats by a choline‐deficient diet. The animals were randomized into three groups: I (G1) and II (G2), n=6 each ‐ fed with a choline‐deficient diet for four and twelve weeks respectively; Group III (control‐G3; n=6) ‐ fed with a standard diet for twelve weeks. Samples of plasma and liver were submitted to biochemical, histological and oxidative stress analysis. Variables measured included serum levels of aminotransferases (AST, ALT), cholesterol and triglycerides. Oxidative stress was measured by lucigenin‐enhanced luminescence and the concentration of hydroperoxides (CE‐OOH‐cholesteryl ester) in the liver tissue. Results: We observed moderate macro‐ and microvesicular fatty change in periportal zones G1 and G2 as compared to controls (G3). In G2, fatty change was more severe. The inflammatory infiltrate was scanty and no fibrosis was seen in any group. There was a significant increase of AST and triglycerides in G1 and G2 as compared to control group G3. The lucigenin‐amplified luminescence (cpm/mg/min × 103) was significantly increased in G1 (1393±790) and G2 (7191±500) as compared to controls (513±170),pp<0.05. Conclusion: 1) Oxidative stress was found to be increased in experimental liver steatosis; 2) The production of reactive oxygen species was accentuated when liver steatosis was more severe; 3) The alterations produced by oxidative stress could be an important step in the pathogenesis of nonalcoholic fatty liver disease.