Genetic Control of the Specificity of Anti-DNP Antibodies
Open Access
- 1 July 1970
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 105 (1) , 314-321
- https://doi.org/10.4049/jimmunol.105.1.314
Abstract
Differences in the fine specificity of anti-2,4-dinitrophenyl (DNP) antibodies produced by different inbred mouse strains were demonstrated. These differences are expressed in terms of the relative capacity of a given nitrophenyl ligand to inhibit the binding of 3H-DNP-∈-aminocaproic acid (3H-DNP-EACA) by anti-DNP antibodies. The most prominent differences concerned the relative affinity of AKR and of BALB/c anti-DNP antibodies for 2,4-dinitrophenol and 2,4-dinitroaniline and the relative affinity of AL/N and C57BL/6 anti-DNP antibodies for picryl-ε-aminocaproic acid. These strain-specific fine specificity patterns could not be explained by systematic differences in average association constant for 3H-DNP-EACA. The specificity patterns of anti-DNP keyhole limpet hemocycanin and anti-DNP bovine γ globulin antibodies produced by a given strain were generally similar. The differences in fine specificity pattern between AKR and BALB/c anti-DNP antibodies were noted in antisera collected at various times in the course of the immune response. Strain-determined variation in the fine specificity of anti-DNP antibodies may present a model system for studying the genetic control of antibody specificity.Keywords
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