On the Synthesis of a32P-Labelled Edman Reagent for the Sensitive Identification of Amino-Acid Derivatives

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Abstract
A phosphorus-32 containing derivative of phenylisothiocyanate was prepared to increase the sensitivity of amino-acid sequence determination. The respective compound 2-(4-isothiocyanatophenoxy)-1,3,2-dioxaphosphinane-2-oxide showed about the same reactivity, stability and polarity as the Edman reagent itself. A repetitive yield of 94% was obtained in the stepwise degradation of insulin B chain using a solid phase sequencer. Synthesis of this radioactive reagent was achieved within 5 h but with a specific activity of 1 Ci/mol. Eight amino acids were reacted with the 32P-labeled reagent and identified by autoradiography after 2-dimensional TLC.

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