Identification of Human Papillomavirus Type 16 E7 Protein by Monoclonal Antibodies
Open Access
- 1 November 1987
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 68 (11) , 2933-2938
- https://doi.org/10.1099/0022-1317-68-11-2933
Abstract
A number of human papillomavirus (HPV) type 16 proteins have recently been identified in human cervical carcinoma cell lines using polyclonal antisera against papillomavirus gene products expressed in Escherichia coli. E7 protein has been found to be the most abundant papillomavirus protein in these cells. Here we describe a panel of monoclonal antibodies recognizing a 15 K Mr non-glycosylated cytoplasmic HPV-16 E7 protein. One of the antibodies cross-reacted with HPV-18 E7 protein.This publication has 12 references indexed in Scilit:
- Expression of the human papillomavirus type 18 E7 gene by a cassette-vector system for the transcription and translation of open reading frames in eukaryotic cells.The EMBO Journal, 1987
- Transcription of human papillomavirus type 16 early genes in a cervical cancer and a cancer-derived cell line and identification of the E7 protein.Proceedings of the National Academy of Sciences, 1986
- Transient replication of bovine papilloma virus type 1 plasmids: cis and trans requirements.Proceedings of the National Academy of Sciences, 1986
- A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer.The EMBO Journal, 1984
- IDENTIFICATION OF HUMAN PAPILLOMAVIRUS IN CERVICAL SWABS BY DEOXYRIBONUCLEIC-ACID INSITU HYBRIDIZATION1984
- The nuclear matrix: Three-dimensional architecture and protein compositionCell, 1982
- Structure of Ia antigens: identification of dimeric complexes formed by the invariant chain.The Journal of Immunology, 1982
- A New Mouse Myeloma Cell Line that Has Lost Immunoglobulin Expression but Permits the Construction of Antibody-Secreting Hybrid Cell LinesThe Journal of Immunology, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- New Human Tumor Cell LinesPublished by Springer Nature ,1975