Density gradient centrifugation (DGC) in Percoll for isolation and characterization of Oscillatoria spp. from Tjeukemeer

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Abstract
SUMMARY. A high degree of separation was achieved in isolating Oscillatoria redekei and O. agardhii separately from Tjeukemeer seston by density gradient centrifugation (DGC) in Percoll. When diatoms were abundant, however, a pre‐incubation in settling cyhnders was applied to obtain an optimal separation of O. redekei from diatoms. One major band dominated by O. redekei was formed in the gradients. O. agardhii showed a varying distribution over three different bands and its banding pattern seemed to be related to the centrifugation resistance of its gas vacuoles and the turgor pressure. Pigment ratios of O. agardhii were not affected by DGC in Percoll.