BIOSYNTHESIS IN VITRO OF 18-HYDROXY-11-DEOXYCORTICOSTERONE BY THE INTERRENAL TISSUE OF THE RAINBOW TROUT

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Abstract
Phillips, Holmes & Bondy (1959) identified aldosterone in the blood of the Pacific salmon, but Idler, Ronald & Schmidt (1959) were unable to detect this steroid in the same material. After incubation of [16-3H]progesterone with the head kidney of the killifish, biosynthesis of aldosterone could be demonstrated (Phillips & Mulrow, 1959). Recently, Sandor, Vinson, Chester Jones, Henderson & Whitehouse (1966), in incubation studies, failed to detect 18-hydroxylase activity in adrenocortical tissue of the eel. The present investigation was carried out to examine the 18-hydroxylation of steroids by the interrenal tissue of the rainbow trout (Salmo gairdneri). The head kidneys containing interrenal tissue were isolated from 3-yr.-old rainbow trout and were homogenized in Krebs—Ringer phosphate-bicarbonate solution (pH 7·0) in a loose-fitting Teflon-glass homogenizer at 0–5°. The homogenate (10 ml.) containing head kidney tissue (5·0 g.) and NADPH (12 μm) as cofactor was incubated with [4-14C]deoxycorticosterone (5

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