RIBONUCLEIC ACID POLYMERASE OF AZOTOBACTER VINELANDII, I. PRIMING BY POLYRIBONUCLEOTIDES
- 1 January 1963
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 49 (1) , 88-94
- https://doi.org/10.1073/pnas.49.1.88
Abstract
The RNA polymerase, from extracts of A. vinelandii, used polyribonucleotides (poly-A, poly-U, poly-C) in addition to DNA as primers. Priming by homopolynucleo-tides directed the incorporation of the complementary nucleotides. The competition between the various primers suggested that a single enzyme is involved. Polyfluorouridylate was an effective inhibitor of both the DNA- and polyribonucleotide-primed reactions Actinomycin-D which inhibits the reaction primed by DNA had no appreciable effect on the polyribonucleotide-primed reaction.Keywords
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